The validated UHPLC method was demonstrated to be precise, accurate, and robust for the simultaneous determination of vitamins D3 (CT and CHL) and K2 (MK-4 and MK-7) in dietary supplements. The validation results indicated that the method had reasonable linearity ( R 2 ≥ 0.9990), good recovery (>82%), satisfactory intra-day precision (≤1.9%) and inter-day precision (≤3.5%), and high selectivity and specificity. The limits of detection and limits of quantification for the target nutritional compounds ranged between 0.04–0.05 µg/mL, respectively. The overall runtime per sample was above 35 min, with the retention times of 2.40, 6.59, 7.06, and 32.6 min for vitamin D3 (CT and CHL) and vitamin K2 (MK-4 and MK-7), respectively. In this regard, the simultaneous determination of vitamin D3 (calcitriol-CT and cholecalciferol-CHL) and K2 (menaquinone-4-MK-4 and menaquinone-7-MK-7) in dietary supplements was developed by using ultra-high-pressure liquid chromatography (UHPLC). Accurate determination of vitamins is important for the improvement of dietary supplement quality and nutrition assessments. The tasteless liquid formula can easily be combined with any beverage.The content and composition of dietary supplements is of great interest due to their increasing consumption and variety of available brand offered in the market. Liquid Vitamin D3 with K2 includes 10 mcg of MenaQ7® PRO, the most widely studied form of vitamin K2 as MK-7, and 1,000 IU of vitamin D3 per serving. Leon Schurgers, world-renowned expert in vitamin K2 as MK-7 research for cardiovascular and bone health, and his team of research scientists have conducted over 15 clinical trials on MenaQ7® PRO through the University of Maastricht.
The addition of MenaQ7® PRO to this formula is backed by extensive research. Therefore, vitamin K and vitamin D not only share similar qualities, but they also act in synergy within the body. In addition, vitamin K has been found to help maintain bone mineral density by decreasing the activity of osteoclasts, a cell that breaks down the bone matrix. Through its activation of the protein osteocalcin, vitamin K helps to bind newly absorbed calcium to the mineral matrix in bone.
A group of naturally occurring and structurally similar, fat-soluble vitamins, vitamin K is essential for the proper utilization of calcium. New research is focusing on the synergistic relationship between vitamin K (specifically, vitamin K2) and vitamin D3, especially in terms of bone strength and cardiovascular health. A growing body of research shows that when it comes to bone health, ensuring optimal intake of vitamin K is a critical piece of the puzzle.
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